Polarographic Catalytic Waves and Reactivity of Sulfhydryl Groups in Bovine Plasma Albumin Solutions Before and after Ultraviolet Irradiation in the Absence and Presence of Radiation Protective Agents. Viscosity and Optical Rotation

摘要

Polarographic studies of several antiradiation drugs were performed and it was found that although the mercaptans be have alike a remarkable difference exists in the polarography of their disulfides. The catalytic mercaptan waves were studied. Under favorable experimental conditions these waves can be applied to the quantitative determination of traces of mercaptans, disulfides, and thiosulfates, and in the study of the changes which occur in albumin upon ultraviolet (UV) irradiation in the absence and presence of radiation protective agents. The experiments show that antiradiation drugs can preclude denaturation of albumin through UV irradiation. The effects of UV irradiation prior to investigating the changes in albumin upon exposure to ionizing irradiation were also studied since the reactions occurring during UV irradiation probably precede those which occur upon exposure to high energy irradiation. A quantitative study of structural changes in albumin is the determination of the reactivity of sulfhydryl and disulfide groups in relation to physical changes, such as viscosity and optical rotation of the protein upon exposure to radiation in the presence and absence of radiation protective agents. In this way it is possible to obtain a better insight into the mechanism of the radiation effects on protein and of the action of radiation protective agents in biological systems. (ASUTHOR)