METHODS FOR DETERMINING PROTEIN TARGETS OF A LIGAND WITHOUT LIGAND DERIVATIZATION

摘要

The present invention concerns a method of determining whether a non-purified sample contains a cellular binding partner, the target protein, of a ligand of interest comprising the steps of (a) preparing a 'ligand specific affinity matrix' by immobilizing the ligand using its inherent intermolecular forces for non-covalent binding on a surface that provides possibilities of forming complementary non- covalent bonds with the ligand, (b) confirming immobilization of ligand on surface offering complementary non-covalent interactions by measuring amount of ligand present in wash solvent used for surface washing; and (c) analyzing biological samples using ligand specific affinity matrix.