A NOVEL SCREENING METHOD FOR SCREENING OF ANTI-HIV MOLECULES

摘要

The present invention relates to a novel screening method for screening of anti-HIV therapeutics. In particular, the present invention relates to reporter gene constructs for the detection of the HIV Nef and host PACS-1 protein interaction. Furthermore, the invention relates to a functional interaction for Nef-PACS-1 proteins prepared in a recombinant manner, a method for identifying of Nef-PACS interaction which causes activation of pathway to down regulate MHC class I molecules from cell surface presumably causing immune evasion for HIV in infected cells. The reporter gene construct according to the present invention, after it had been introduced into cells, in the presence of HIV Nef and host PACS-1 proteins result in the expression of reporter luciferase protein which may be used for quantitative/qualitative interaction of HIV Nef and host PACS-1 protein. Furthermore, the invention was used to identify the inhibitor for the interaction of Nef-PACS in the cell.