Obtaining spectral images for the measurement of nuclear pathological characteristics in cancer cells prepared for in situ analysis

摘要

Diagnostic method of multivariate cancer, the method comprising: a. obtain molecular marker data from a single sample of a subject comprising a single cell or population of cells from a tissue, in which the molecular marker is a genetic reorganization, b. obtain quantitative data of the cell morphology of the same individual cell or population of cells used in step (a), in which the data of markers of the cell morphology are the nuclear size, the nuclear form and the DNA content, c . carrying out a multivariable analysis of said single sample to generate a multivariable analysis data set comprising quantitative data of cell morphology markers of stage (b) and molecular marker data of stage (a), d. compare the multivariable analysis data obtained in step (c) with a reference multivariable analysis data set created by obtaining both molecular marker data and quantitative cellular morphology data from cancer and non-cancer cell samples obtained from individuals with a known clinical outcome, in which the molecular marker is a genetic reorganization and the data of cell morphology markers are nuclear size, nuclear form and DNA content, and e. predict a clinical outcome defined by specific combinations of cellular morphology marker data and molecular marker data statistically associated with the progression, incidence and metastasis of cancer or other clinical outcome determinant observed in the multivariable baseline analysis data set.