A device for microfluidic neural growing multiple compartments comprising: a top of the device transparent polymer optically with two separate compartments separated by a zone of physical barrier formed at the top of the device, wherein said upper device is sealed to a plane substrate to create a waterproof seal, and where two separate compartments have openings at each end to serve as inputs for the load cells and cell media; and a plurality of Micron size channel embedded in the wall, wherein each of said plurality of Micron size channel has a width and height and length, where the length traverses the distance from the barrier zone between the separate compartments wherein said plurality of micron size channel is sized to maintain a hydrostatic pressure difference between the compartments resulting in a fluidic isolation of one of the independent compartments.
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