PHARMACEUTICAL COMPOSITION INCLUDING CELL PENETRABLE PRAS40 FUSION PROTEIN FOR PREVENTING AND TREATING PARKINSONS DISEASE

摘要

The Parkinsons disease is a neurodegenerative disease in which dopamine neurons are gradually removed from the substantia nigra of the brain. The inventors have clarified that a PRAS40 fusion protein can protect dopamine neurons from oxidation stress in SH-SY5Y neuroblastoma cells and Parkinsons disease animal models. The PRAS40 fusion protein has been confirmed to be able to smoothly move into SH-SY5Y cells and into the substantia nigra of the brain through a Western blot analysis. The PRAS40 fusion protein remarkably inhibits DNA fragment and production of active oxygen species induced by MPP^+, and the survival of SH-SY5Y cells is eventually induced. In addition, immunohistochemical analyzing data using TH antibody and cresyl violet coloration shows that the PRAS40 fusion protein remarkably protects dopamine cells in the substantia nigra with respect to oxidation stress such as MPTP. Accordingly, the PRAS40 fusion protein can be used in preventing and treating the Parkinsons disease.;COPYRIGHT KIPO 2017