SOX Non-viral minicircle vector encoding SOX genes and method for preparing thereof

摘要

The present invention relates to a non-viral minicirclevector expressing a SOX gene, a stem cell into which said vector is introduced, a pharmaceutical composition for preventing or treating cartilage diseases comprising said stem cell, and a method for producing said vector. When transforming mesenchymal stem cells with MC / SOX-Trio or MC / SOX-Duo, which is a nonviral mini-circle vector according to the present invention, it is essential to induce chondrocyte differentiation using mesenchymal stem cells The need for expensive growth factors that have been used can be completely ruled out. Therefore, the transformed mesenchymal stem cells can differentiate into chondrocyte cells in vivo, thereby simplifying the complex steps of transplanting cells after induction of differentiation. In addition, unlike conventional vector systems in which foreign genes derived from antibiotic resistance genes and other bacteria are simultaneously transfected after transformation, two or three kinds of SOX genes necessary for differentiation into chondrocytes are regulated under a single promoter, Since the introduction of genes is minimized, it can be used as the most favorable form of nonviral vector system for clinical application of stem cell-gene therapy.