NOVEL 7ALPHA-HYDROXYSTEROID DEHYDROGENASE KNOCKOUT MUTANTS AND USE THEREOF

摘要

The invention relates to 12 -hydroxysteroid dehydrogenases, nucleic acid sequences coding for the same, expression cassettes and vectors, recombinant microorganisms containing the corresponding coding nucleic acid sequences, a method for producing said 12 -hydroxysteroid dehydrogenases, a method for enzymatic oxidation of 12 -hydroxysteroids using said enzyme, a method for enzymatic reduction of 12-ketosteroids using said enzyme, a method for qualitative or quantitative determination of 12-ketosteroids and/or 12-hydroxysteroids using said 12-hydroxysteroid dehydrogenases and a method for production of ursodesoxycholic acid, comprising the enzyme-catalyzed cholic acid oxidation using said 12 -hydroxysteroid dehydrogenases.