Embodiments of the present invention provide an electroporation system that includes at least two electrodes adjacent to each other configured to be inserted into a biological tissue for electroporation treatment; A pulse generator electrically connected to a probe, wherein one or more electrical pulse sequences are used to drive the electroporation probe and transmit current to the probe to cause unevenness in biological tissue near the probe electrode The pulse generator configured to induce an electric field. Treated tissue can be targeted by controlling parameters of probe configuration, carrier solution properties and electroporation pulse sequence to achieve predictable electroporation outcomes. This electroporation control method can also reduce the potentially harmful effects of electroporation procedures. [Selection] Figure 2b
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