Methods and compositions related to functional polypeptides incorporated into heterologous protein scaffolds

摘要

The present invention provides methods for making functional derivatives of effector polypeptides (eg, hormones and receptor ligands) incorporated into different protein scaffolds (eg, antibody scaffolds). This method modifies an effector polypeptide with a combinatorial library of terminal linker sequences, inserts the modified sequence into the host backbone, and then functional derivatives from the library of modified polypeptide sequences incorporated into the host backbone. Including selecting. By way of example, the present invention also provides certain functional derivatives of leptin, scFSH and sc relaxin incorporated into the antibody backbone, and therapeutic applications of such functional fusion molecules. [Selection] Figure 1