Methods to modify a sequence of nucleotides in the genome of a plant cell, produce a plant, produce callous plant tissue that has a modified nucleotide sequence in its genome without the use of a selectable marker, and plant progeny

摘要

These are compositions and methods to modify a sequence of nucleotides in the genome of a plant cell without the use of a selectable marker. The methods and compositions employ a guidance -polynucleotide /endonuclease system to produce a dual filament rupture at a target site located in a nucleotide sequence,And plant cells are obtained without the use of a selectable marker,And to provide an effective system for modifying target sites within the genome of a plant,Plant cell or seed. Preparations and methods for producing a plant cell are also provided,Callous or plant tissue that has a sequence of nucleotides modified in its genome,Without the use of a selectable marker.