method for modifying a target sequence in the genome of a plant cell; method for editing a nucleotide sequence in the genome of a plant cell; method for simultaneously modifying multiple target sequences in the genome of a plant cell; method for modifying a DNA target sequence in a plant cell genome and polynucleotide modification model

摘要

These are compositions and methods for genome modification of a target sequence in the genome of a plant or plant cell, for genome editing of a nucleotide sequence in the genome of a plant or plant cell and / or for inserting or deleting a polynucleotide of interest in or of the genome of a plant. The methods and compositions employ a guide / cpf1 endonuclease polynucleotide system to provide an effective system for modifying or altering target sequences within the genome of a plant, plant cell or seed. Also provided are guide polynucleotide / cpf1 endonuclease complexes, guide polynucleotides, plant optimized cpf1 endonuclease sequences, recombinant DNA constructs comprising plant optimized cpf1 endonuclease genes, and combinations thereof.