MICROORGANISM STRAIN AND METHOD FOR ANTIBIOTIC-FREE, FERMENTATIVE PREPARATION OF LOW MOLECULAR WEIGHT SUBSTANCES AND PROTEINS

摘要

The invention relates to a microorganism strain and a method for antibiotic-free fermentative preparation of low molecular weight substances and proteins. The microorganism strain for producing low molecular weight substances or proteins contains in its genome a mutation in a gene which brings about an auxotrophy in the strain. It further contains a production plasmid coding an enzyme for production of a low molecular weight substance or a recombinant protein and a functional copy of the gene whose chromosomal inactivation brings about the auxotrophy wherein the auxotrophy is a non-feedable auxotrophy.