Study on Preparation of the Low-Molecular-Weight Chitosan Using Cellulase

摘要

The degradation of chitosan ( DD of 72.05%) with aid of cellulase was carried out under the conditions of 45℃, pH 5.0 and a ratio of 1:15( chitosan/enzyme). The results showed cellulase could degrade chitosan efficiently. Viscosity of chitosan was decreased very quickly and reducing sugar released was increased with time during degradation. By using the membrane, the separation of the hydrolysis mixture was studied. Rejection of protein can be reached to be 99.74%.65.9% of low-molecular-weight chitosans was less than 2 kDa. Solubility of low-molecular-weight chitosan was found to be better than chitosan and transmittance could reach to be more than 95 % in entire range of pH 1 ～ 13.