SITE-DIRECTED EDITING METHOD FOR PLANT GENOME BASED ON CPF1

摘要

Disclosed is a site-directed editing method for plant genome based on Cpf1. In particular, disclosed are a nucleic acid construct, vector or vector combination for plant genome site-directed editing based on Cpf1 (AsCpf1, FnCpf1, LbCpf1), and a site-directed editing method for plant genome. In particular, the nucleic acid construct comprises a first expression cassette and an optional second expression cassette. The first expression cassette is a Cpf1-NLS fusion protein expression cassette. The second expression cassette is a crRNA expression cassette. With the method, the single-gene knockout, multiple-gene knockout or homologous recombination and directional insertion of foreign fragments can be performed simply and efficiently at a predetermined plant genomic site.