GENETIC CONSTRUCT BASED ON CRISPR/CAS9 GENOME EDITING SYSTEM ENCODING CAS9 NUCLEASE FOR GENOME EDITING OF MONOCOTYLEDONOUS GRAIN CROPS BASED ON DOUBLE SELECTION OF PLANTS

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to the field of biotechnology, particularly, to a genetic construct based on the CRISPR/Cas9 genome editing system encoding the Cas9 nuclease, containing the bar gene-selectable marker and the gfp reporter gene.;EFFECT: invention ensures efficient selection of modified plants without additional molecular and biological testing. ;1 cl, 6 dwg, 2 ex