PROTEIN (ENZYME) FOR L-THREONINE DEHYDROGENATION, AND METHOD FOR SCREENING AND METHOD FOR PREPARING PROTEINS HAVING TARGET ACTIVITY

摘要

The present invention relates to a protein having L-threonine dehydrogenase activity (including for L-threonine analysis), a method for screening said protein, a method for preparing said protein, a method for screening and a method for preparing proteins having a target activity, a method for preparing a novel synthetic protein having a target enzyme activity such as L-threonine dehydrogenase activity, and a method for analyzing L-threonine. Provided is a protein (enzyme) having L-threonine dehydrogenation activity suited for use in L-threonine analysis, the protein having higher affinity than in the past for NAD⁺ as well as high enzyme stability. Provided are a novel method for screening enzymes having a target activity on the basis of amino acid sequence data, irrespective of the enzyme name registered in a database, and a method for preparing enzymes screened by this method. Provided is a method for preparing proteins having a target enzyme activity, not limited to existing proteins. Provided is a method for analyzing L-threonine using newly discovered proteins having L-threonine dehydrogenation activity.