PD-L1 KNOCKOUT CAR-T TRANSGENIC VECTOR FOR SUPPRESSING IMMUNE ESCAPE, PREPARATION METHOD THEREOF, AND APPLICATION OF SAME

摘要

Provided is a PD-L1 knockout CAR-T transgenic vector for suppressing immune escape, comprising: an ampicillin resistant (AmpR) sequence as indicated by SEQ ID NO. 1; a prokaryotic replicon pUC Ori sequence as indicated by SEQ ID NO. 2; a viral replicon SV40 Ori sequence as indicated by SEQ ID NO. 3; an enhanced woodchuck hepatitis virus posttranscriptional regulatory element (eWPRE) as indicated by SEQ ID NO. 11; a human EF1α promoter as indicated by SEQ ID NO. 12; a lentivirus packaging cis-element for lentivirus packaging; human PD-L1 humanized single-chain antibodies PDL1scFv1 as indicated by SEQ ID NO. 21, PDL1scFv2 as indicated by SEQ ID NO. 22, or PDL1scFv3 as indicated by SEQ ID NO. 23; an IRES ribosome binding sequence as indicated by SEQ ID NO. 25; an IL-6 signal peptide as indicated by SEQ ID NO. 26; a humanized antibody Fc fragment as indicated by SEQ ID NO. 27; and a second generation or third generation chimeric antigen receptor (CAR) combining the functions of identification, transfer, and activation. Further provided is a method for preparing the vector and an application of the vector in preparing a pharmaceutical drug for suppressing immune escape.