PD-L1 KNOCKOUT CAR-T TRANSGENIC VECTOR FOR SUPPRESSING IMMUNE ESCAPE, PREPARATION METHOD THEREOF, AND APPLICATION OF SAME

摘要

The invention discloses a PDL1 block CAR-T transgenic vector for suppressing immune escape, including: AmpR sequence containing ampicillin resistance gene (SEQ ID NO.1); prokaryotic replicon pUC Ori sequence (SEQ ID NO.2); virus replicon SV40 Ori sequence (SEQ ID NO.3); eWPRE enhanced posttranscriptional regulatory element of hepatitis B virus (SEQ ID NO.11); human EF1a promoter (SEQ ID NO.12); lentiviral packaging cis-elements for lentiviral packaging; humanized single-chain antibody fragment PDL1scFv1 (SEQ ID NO.21), PDL1scFv2 (SEQ ID NO.22), or PDL1scFv3 (SEQ ID NO.23) of human PDL1; IRES ribosome binding sequence (SEQ ID NO.25); IL6 signal peptide (SEQ ID NO.26); human antibody Fc segment (SEQ ID NO.27); and the chimeric antigen receptors of the second or third generation CAR for integrating recognition, transmission and initiation. Also, the invention discloses the preparation method of the vector and its application in the preparation of anti-immune escape drugs.