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PDL1 block CAR-T transgenic vector for suppressing immune escape, preparation method thereof, and application of the same
PDL1 block CAR-T transgenic vector for suppressing immune escape, preparation method thereof, and application of the same
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机译:用于抑制免疫逃逸的pdl1阻滞CAR-T转基因载体及其制备方法和应用
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摘要
A PDL1 block CAR-T transgenic vector for suppressing immune escape includes: AmpR sequence containing ampicillin resistance gene (SEQ ID NO: 1); prokaryotic replicon pUC Ori sequence (SEQ ID NO: 2); virus replicon SV40 Ori sequence (SEQ ID NO: 3); eWPRE enhanced posttranscriptional regulatory element of hepatitis B virus (SEQ ID NO: 11); human EF1a promoter (SEQ ID NO: 12); lentiviral packaging cis-elements for lentiviral packaging; humanized single-chain antibody fragment PDL1scFv1 (SEQ ID NO: 21), PDL1scFv2 (SEQ ID NO: 22), or PDL1scFv3 (SEQ ID NO: 23) of human PDL1; IRES ribosome binding sequence (SEQ ID NO: 25); IL6 signal peptide (SEQ ID NO: 26); human antibody Fc segment (SEQ ID NO: 27); and chimeric antigen receptors of the second or third generation CAR for integrating recognition, transmission and initiation. A preparation method of the PDL1 block CAR-T transgenic vector and an application thereof in a preparation of anti-immune escape drugs.
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机译:用于抑制免疫逃逸的PDL1嵌段CAR-T转基因载体包括:含有氨苄青霉素抗性基因的AmpR序列(SEQ ID NO:1);和原核复制子pUC Ori序列(SEQ ID NO:2);病毒复制子SV40 Ori序列(SEQ ID NO:3); eWPRE增强了乙型肝炎病毒的转录后调控元件(SEQ ID NO:11);人EF1a启动子(SEQ ID NO:12);慢病毒包装用于慢病毒包装的顺式元素;人源化PDL1的单链抗体片段PDL1scFv1(SEQ ID NO:21),PDL1scFv2(SEQ ID NO:22)或PDL1scFv3(SEQ ID NO:23); IRES核糖体结合序列(SEQ ID NO:25); IL6信号肽(SEQ ID NO:26);人抗体Fc片段(SEQ ID NO:27);第二或第三代CAR的嵌合抗原受体,用于整合识别,传递和起始。 PDL1阻滞CAR-T转基因载体的制备方法及其在制备抗免疫逃逸药物中的应用。
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