The present invention relates to a method for purely separating corneal limbal stem cells and a method for mass culturing the corneal limbal stem cells. The present invention relates to a method for pure separation and mass culture of corneal limbal stem cells, As well as maintaining the stem cell ability of the stem cellsin vitroIt is expected that the present invention can be practically commercialized in various fields such as artificial corneal preparation, corneal regeneration, or treatment of corneal injury, because it enables easy mass cultivation of corneal limbal epithelial stem cells.
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