-32 B Antiviral compositions against hepatitis B virus comprising interleukin-32 as an active ingredient

摘要

The present invention relates to an antiviral composition against the hepatitis B virus, comprising interleukin-32 (IL-32) as an active ingredient, which is an inhibitor inhibiting the expression or activity of hepatocyte nuclear factor 4 alpha (HNF4α) or hepatocyte nuclear factor 1 alpha (HNF1α), and to a method for screening an antiviral material against the virus. The present inventors have found that the secretion of IL-32 is induced by TNF-α and IFN-γ cytokines that are secreted from the infected cells when host cells are infected with the hepatitis B virus; and the secreted IL-32 increases the phosphorylation of ERK1/2, thereby reducing the expression of HNF4α and HNF1α, which are transcription factors of the host involved in the transcription of the viral genes, and thus the present inventors have experimentally confirmed the inhibitory effect on DNA cloning and gene expression of the virus, and have identified the antiviral effects of IL-32 mediated hepatitis B virus and a molecular mechanism thereof. Accordingly, the antiviral ability of IL-32 against the hepatitis B virus, newly identified according to the present invention, provides a new understanding for the development of treatment agents for the virus, and may be beneficially used in the development of antiviral therapeutic agents.