The Collagen Hydrolysate Manufacturing Method and the Collagen Tripeptides Manufacturing Method Using the Collagen Hydrolysate

摘要

The method for producing collagenase according to the present invention comprises a first step of centrifuging the strain of Bacillus subtilis, a second step of concentrating the supernatant separated by centrifugation, and a third step of purifying by using ion exchange chromatography The method comprising the steps of: mixing the pretreated child with water at a weight ratio of 2: 8; and performing a second step of heat-treating the mixture at 90C for 5 hours A third step of adding collagenolytic enzyme prepared by the above method and decomposing at a temperature of 35C for 12 hours, a fourth step of removing foreign matters through centrifugation in the third step, A fifth step of purifying the above components by ion exchange chromatography, a sixth step of concentrating the purified component, a seventh step of purifying the concentrated component using activated carbon, And an eighth step of filtering the component by using a filter.;