The manufacturing method of decomposition of collagen enzyme and the manufacturing method for utilizing the collagen tripeptide, wherein

摘要

A method for preparing collagenase of the present invention comprises: a first step for centrifuging a Bacillus subtilis strain; a second step for concentrating the centrifuged supernatant; and a third step for purifying the supernatant using ion exchange chromatography. A method for preparing a collagen tripeptide comprises: a first step for mixing pre-treated fish scales and water at a weight ratio of 2:8; a second step for heat-treating the mixture at 90 DEG C for 5 hours; a third step for adding the collagenase prepared by the above-described method, followed by degradation at 35 DEG C for12 hours; a fourth step for removing foreign materials from the component in the third step through centrifugation; a fifth step for purifying the component by ion exchange chromatography; a sixth step for concentrating the purified component; a seventh step for purifying the concentrated component using activated carbon; and an eighth step for removing microorganisms from the component using a filter.