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FUNCTIONAL COLLAGEN HYDROLYSATE MANUFACTURING METHOD

机译:功能性胶原蛋白水解物的制造方法

摘要

FIELD: food industry.;SUBSTANCE: invention is related to food industry, in particular, to meat processing branch. The method involves collection of meat trimming wastes, removal of visible fat and muscular tissue remains, washing in 30-35°C flowing water during 5-10 min; milled in a mincer with 2-3 mm diametre holes, the milled mass treatment with enzyme preparations. At the first stage one applies Neutrase 1.5 MG general proteolytic activity enzyme preparation for removal of ballast substances; to provide for that one prepares a water solution of the preparation and adds it to the milled mass with a water duty of 1:2, the dosage being 5 proteolytic activity units per 1 g of raw material. Treatment proceeds during 2-2.5 h at a temperature of 45-55°C and pH=6.8-7.2. After treatment the solid fraction is washed with water for removal of hydrolysis products and the enzyme and separated by way of centrifugation at a 83 rps rotation rate during 5 minutes. Then one proceeds with milling on a homogeniser; added to the milled mass is a water solution of "Food Collagenase" collagenolytic activity enzyme preparation with a water duty of 1:2, taken in a quantity of 6 proteolytic activity units per 1 g of raw material. Treatment proceeds during 2.5-3 h at a temperature of 37-40°C and pH=6.8-7.2. Solid fraction is washed with water for removal of hydrolysis products and the enzyme and separated by way of centrifugation at a 83 rps rotation rate. The produced collagen hydrolysate is packaged into polyethylene bags and frozen.;EFFECT: method ensures enhancement of the functional collagen product quality and output, technological process intensification, and collagen native structure preservation.;1 tbl, 2 ex
机译:技术领域:本发明涉及食品工业,尤其涉及肉类加工部门。该方法包括收集切肉废物,去除可见的脂肪和肌肉组织残留物,在30-35°C的流水中洗涤5-10分钟;在具有2-3毫米直径孔的切碎机中研磨,然后用酶制剂进行研磨处理。在第一阶段,采用Neutrase 1.5 MG一般蛋白水解活性酶制剂来去除压舱物;提供一种用于制备该制剂的水溶液并将其以1∶2的水负荷添加到研磨的物料中的方法,该剂量是每1g原料5个蛋白水解活性单位。处理在45-55°C和pH = 6.8-7.2的温度下进行2-2.5小时。处理后,将固体级分用水洗涤以除去水解产物和酶,并在5分钟内通过以83 rps的旋转速率离心分离。然后,在均质机上进行研​​磨。加入到研磨的物料中的是“食品胶原酶”胶原蛋白水解活性酶制剂的水溶液,其水负荷为1:2,每1 g原料中的蛋白水解活性单位为6。处理在温度37-40°C和pH = 6.8-7.2的2.5-3小时内进行。用水洗涤固体部分以除去水解产物和酶,并通过离心以83 rps的旋转速率进行分离。产生的胶原蛋白水解产物包装到聚乙烯袋中并冷冻。效果:该方法可确保功能性胶原蛋白产品的质量和产量提高,工艺流程得到加强以及胶原蛋白天然结构的保存。1tbl,2 ex

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