PNA Ct Method of Solving Nucleic Acid Detection Problem Based on Threshold CycleCt of Polymerase Chain Reaction Using Peptide Nucleic Acid Probe

摘要

The present invention relates to a method for solving the problem of nucleic acid detection according to a critical cycle (Ct) of a real-time PCR reaction using a PNA probe, and more particularly to a method for detecting a nucleic acid using a reporter and a small photon quencher, and a method for detecting and quantifying a target nucleic acid for solving the problem of detecting a target nucleic acid according to a threshold cycle (Ct) using the method, and a PNA probe To a kit for detection and quantification of a target nucleic acid.When the detection and quantification method of a target nucleic acid using a reporter and a quencher-coupled PNA probe according to the present invention is used, the critical cycle (Ct ) Value, it is possible to improve the accuracy of detecting the target nucleic acid or the target gene and to read out the quantitative result, and it is possible to reduce economic, social and medical costs.;