METHOD FOR SOLVING PROBLEM OF NUCLEIC ACID DETECTION DUE TO THRESHOLD CYCLE (CT) OF REAL-TIME POLYMERASE CHAIN REACTION USING PNA PROBE

摘要

The present invention relates to a method for solving the problem of nucleic acid detection due to a threshold cycle (Ct) of the real-time polymerase chain reaction using a PNA probe and, more specifically, to a method for analyzing a melting curve using a PNA probe to which a reporter and a quencher for detecting a target nucleic acid are bound, to a method for detecting and quantifying a target nucleic acid to solve the problem of the detection of the target nucleic acid due to a threshold cycle (Ct) using the same, and to a kit for detecting and quantifying a target nucleic acid, using the method and comprising a PNA probe. The use of the method for detecting and quantifying a target nucleic acid using a PNA probe, to which a reporter and a quencher are bound, according to the present invention leads to the solving of a technical problem caused by performing the determination on the basis of the threshold cycle (Ct) value of the real-time polymerase chain reaction, which is used in the method for the diagnosis and the identification and analysis of individuals, thereby improving the accuracy in the detection and quantification result read-out of a target nucleic acid or a target gene and reducing economic, social, and medical costs.