BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a dielectrophoresis-based biosensor, and more particularly, A first insulating layer formed on one surface of the substrate; A second insulating layer formed on the first insulating layer; A collecting electrode including a quadrupole electrode embedded between the first insulating layer and the second insulating layer; A collecting electrode including a quadrupole electrode embedded in the second insulating layer; A pair of sensing electrodes formed on the second insulating layer and spaced apart from each other; And an integrating unit connecting the pair of sensing electrodes and integrating a sensing object; To a biosensor based on dielectrophoresis. The present invention can provide a biosensor based on dielectrophoresis capable of improving the sensitivity of a sensor under biological solution of high electrical conductivity and lowering the occurrence of short circuit, thereby enabling a stable sensing operation.
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