LENTIVIRAL VECTOR PSEUDO-TYPED WITH SINDBIS VIRUS ENVELOPE GLYCOPROTEIN

摘要

To provide a lentiviral vector pseudo-typed with a sindbis virus envelope glycoprotein.SOLUTION: The present invention provides a lentiviral vector particles comprising: a Sindbis virus E2 glycoprotein variant; and a lentiviral vector genome comprising a sequence of interest. The lentiviral vector particle comprises: (a) an envelope comprising a Sindbis virus E2 glycoprotein variant; and (b) a lentiviral vector genome comprising a sequence of interest. Here, the E2 glycoprotein variant facilitates infection of dendritic cells by the lentiviral vector particle. The E2 glycoprotein variant has reduced binding to heparan sulfate compared to a reference sequence (HR strain).SELECTED DRAWING: Figure 2