A method that enables selective and specific recognition by changing the functional state of mRNA

摘要

The present invention relates to methods that alter the functional state of any mRNA to allow its selective and specific recognition and subsequent selective manipulation. The present invention describes a universal principle for increasing the specificity and selectivity of molecular target recognition at the nucleic acid level. The principle of specific and selective recognition of nucleic acids is based on the simultaneous recognition of two or more sequences of a target nucleic acid that must be separated from each other by a certain defined distance. Such a method of nucleic acid recognition by specifically recognizing a well-defined sequence of nucleic acids that are separated from each other by a defined distance would allow for stable binding of the interfering construct to the unexpected nucleic acid. Minimizing the possibilities, thereby dramatically increasing the selectivity of target nucleic acid recognition. Specific recognition of a defined sequence of nucleic acids localized at a defined distance from each other is the simultaneous complementary interference of short sequence-specific oligonucleotides interconnected by size-specific polymer linking moieties Achieved by: