Method for producing dendritic cell for stimulating natural killer T (NKT) cell, dendritic cell for stimulating NKT cell, method for producing cell composition comprising dendritic cell for stimulating NKT cell and NKT cell, and NKT cell Cell composition comprising stimulating dendritic cells and NKT cells

摘要

A method for producing a dendritic cell that stimulates NKT cells includes a step of placing a mononuclear cell in a culture vessel and allowing it to stand to allow some of the mononuclear cells to settle on the bottom surface of the vessel, and a bottom surface of the culture vessel. Removing floating cells other than the cells that have settled in the cell, a step of putting a predetermined factor in the culture vessel and differentiating monocytes out of the cells fixed on the bottom surface into immature dendritic cells, and the culture vessel And introducing α-galactosylceramide into mature dendritic cells to induce dendritic cells that stimulate NKT cells.