A method for producing herbicide-tolerant plants using a gene-based Edition system related to herbicide tolerance

摘要

The material belongs to a part of the field of plant genetic engineering. Specifically, it refers to a method to create herbicide resistant plants through basic editing technology, and a method to detect internal gene variation sites that can produce resistance to plant herbicides. In addition, it involves the use of identified internal gene mutation sites in crop reproduction. Claim 1: the best way to produce herbicide tolerant plants is to produce herbicide tolerant plants without genes,This includes the introduction of a system in plants for basic editing of genes related to herbicide resistance in the plant genome. Therefore, RNA guides the combined protein of basic editing to the objective sequence of genes related to herbicide resistance in plants to achieve one or more goals. Further substituting nucleoside in the target sequence, the nucleotide in the target sequence includes at least one (I) a (V) level: a basic editing and combining protein and a guiding RNA; two include a nucleoside sequence, encoding a basic editing and combining protein,and a guide RNA; iii) a base-edition fusion protein, and an expression construct comprising a nucleotide sequence encoding a guide RNA; iv) an expression construct comprising a nucleotide sequence encoding a base-edition fusion protein, and an expression construct comprising a nucleotide sequence encoding a guide RNA; v) an expression construct comprising a nucleotide sequence encoding base-editing fusion protein and a nucleotide sequence encoding guide RNA;The basic editing of this fusion protein includes a CRISPR nuclear domain and a drainage basin, which are treated by core enzymes (such as non active cas9 without core enzymes), Here, the guiding RNA can guide this basic editing and merging protein to an objective gene sequence related to herbicide resistance in the plant genome, in which one or more nucleoside substitutes make the plant herbicide resistance an option, in which the herbicide also includes 1. Screening stage of herbicide resistant plants.