Production of herbicide resistant transgenic rice plant from immature embryos using bioltistic method

摘要

Plasmid pCB1,which contains a bar gene un-der the control of CaMV 35S promoter,hadbeen successfully transformed into immatureembryos of japonica rice(about 12 days afterflower)using blolistic method.The bar genewas isolated from Streptomyces hygroscopicus.It encodes for phosphinothricin acetyl-transferase(PAT)that confers resistance tophosphinothricin(PPT),the active ingredientof the broad—spectrum herbicide Basta.Afterbombardment,the Basta resistant calli