enzymatic hydrolysis process of cellulose to lignocellulosic biomass cellulose that still contains protein, such as straws and grams, can be enzymatically converted to valuable products such as glucose in lower enzyme consumption by: (a) extraction of biomass with water at 15-75 ° C ° C to produce an extracted biomass and an aqueous extract containing protein; (b) mechanically, chemically and / or thermally pre-treating the cellulose-containing biomass, in particular by treating organosolv; and (c) contacting the pretreated biomass in step (b) with: (i) an enzyme capable of hydrolyzing cellulose; and (ii) the protein-containing aqueous extract obtained in step (a).
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