Enzymatic Hydrolysis of Cellulose in Aqueous Ionic Liquids.

摘要

Total enzymatic hydrolysis of the polysaccharides in lignocellulosic biomass tomonosaccharides is currently a focus research area. The monosaccharides obtained from lignocellulose hydrolysis can be used for the production of platform chemicals and biofuels, most notably ethanol. One major challenge in the commercialization of lignocellulosic ethanol production is the recalcitrance of lignocellulosics towards enzymatic hydrolysis, necessitating efficient pretreatment of the lignocellulosic feedstock. Certain ionic liquids (ILs, salts with melting points below100 °C) dissolve cellulose and even lignocellulosic biomass and are as such interesting candidates for pretreatment technology. However, cellulose-dissolving ILs have been found to severely inactivate the hydrolytic enzymes (cellulases) employed in cellulose hydrolysis. This work focuses on elucidating how certain ILs affect the action of cellulases in cellulose hydrolysis. The main emphasis was on the action of purified monocomponent Trichoderma reesei cellulases, but some commercial cellulase preparations were also studied in IL matrices.