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A method for reducing apoptosis in cells and a refrigerated composition comprising nucleated cells stored by said method
A method for reducing apoptosis in cells and a refrigerated composition comprising nucleated cells stored by said method
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机译:减少细胞凋亡的方法和包含通过所述方法储存的有核细胞的冷藏组合物
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摘要
Provided is a method for reducing apoptosis in nucleated cells. The method entails holding nucleated cells in a container and adding a gas containing xenon to the container so that the pressure inside the container reaches between 0.5 to 4.0 Atm above ambient pressure; holding the container at between 0.5 to 4.0 Atm above ambient pressure for a period of time during which the temperature in the container is between 22° C. and 37° C.; lowering the temperature in the container to between 0.1° C. and 10° C. while maintaining the pressure of 0.5 to 4.0 Atm above ambient pressure and holding the container for a period of time; and reducing the pressure in the container to ambient pressure and increasing the temperature to 22° C.-37° C. By performing these steps, the cells undergo less apoptosis than a reference.
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