Method for selective trapping of DNA components containing unpaired bases by MutS protein immobilized in solid phase, and its colorimetric designation and quantitative analysis

摘要

method of selective capture dna containing niesparowane rules by protein muts immobilizowane solid phase in small volumes of solution, is that muts protein is immobilizowane in solid phase, and the dna is to be affixed to the reaction environment in bufferproviding selective binding to dna fragments containing niesparowane rules in the presence of fully complementary dna fragments.how effective recovery of dna captured by immobilizowane solid phase protein muts is that pieces of dna bound by the protein muts immobilizowane in solid phase are efficiently eluting in a manner that allows their direct identification by pcr.a colorimetric indication biotynylowanego dna captured by protein muts is that protein muts accompanied by domain allowing its effective immobilizacju0119 on a constant capture fragments biotynylowanego dna, and its presence is detected by signal as a result, nau0142owi caused hydrolysis substrate enzymatic domain reporterowej coupled with awidynu0105.