the object of the application is a way of przybyszowych cactus aztekium receive myriostigma 'fukuryu hakuyo' originating in cultures in vitro for further cultivation greenhouse test.horticultural crops, horticultural crop production or hobby.way is characterized by the fact that shoots the parent plant sterilizes by soaking 5 - 10 minutes in distilled water with the addition of 10 - 100 ul of detergent, successively washed under running water.clears with hairs, soaking in measures of fungicides by 17 - 18 hours and then washed in 60 - 80% ethanol soaking in sodium hypochlorite solution and concentration of 1 - 2% for 20 - 40 minutes.then washed three times for 5 - 10 minutes in sterile distilled water and dried on sterile blotting paper in the chamber laminarnej,successively shoots cut scalpel on slices of thickness 1 to 10 mm and placed in polar on the medium ms with added agar of 6 - 12 g - dm - 3 and sucrose in quantities of 20 - 50 g - dm - 3 at ph 5.2 - 6.0, then culture in vitro is put in room temperature upward in 24°c + or and relative air humidity of 60 - 80%,zapped it right through the lamps jarzeniowymi of daylight and irradiation intensity quantum 20 - 60 mm - m - 2 - s - 1, for 16 hours a day.
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