METHOD FOR PREPARING CHITOSAN OLIGOSACCHARIDE BY MEANS OF PAPAIN FREEZE DRYING

摘要

The present invention belongs to the field of oligosaccharide preparation, and relates in particular to a method for preparing chitosan oligosaccharide by means of papain freeze drying. The method for preparing chitosan oligosaccharide by means of papain freeze drying comprises steps such as dissolving chitosan in an acetic acid-sodium acetate buffer solution to prepare a chitosan solution, adding a papain solution to the chitosan solution for enzymolysis, performing dialysis on a filtrate by means of a dialysis method after the enzymolysis has finished, heating and concentrating a collected dialysate, and freeze drying. The method for preparing chitosan oligosaccharide by means of papain freeze drying is simple, rapid, efficient and suitable for industrial production, and has good practicality and value for promotion in industry.