USE OF NUCLEIC ACID APTAMER IN ALKALINE PHOSPHATASE HETERODIMER RECOGNITION AND BINDING OR IN TUMOR DETECTION

摘要

Provided is the use of a nucleic acid aptamer in recognizing and binding to an alkaline phosphatase heterodimer. Provided is a nucleic acid aptamer or derivatives thereof, wherein the nucleotide sequence thereof is sequence 1 in the sequence listing. Further provided are a kit comprising the nucleic acid aptamer or derivatives thereof, and a carrier fixing or coupling with the nucleic acid aptamer or derivatives thereof. The nucleic acid aptamer is a single-stranded DNA molecule as shown in sequence 1. The method for capturing and detecting circulating tumor cells that highly express an alkaline phosphatase in peripheral blood, exosomes and free alkaline phosphatases based on nucleic acid aptamer magnetic nanoparticle technology can realize highly selective capturing and detection for target cells, exosomes or free proteins.