Therapeutic Immunoglobulin G4 Design for Improved Bioanalysis and Bioprocessing Properties

摘要

In certain embodiments, the present disclosure includes heavy and light chains, wherein the heavy chain is (a) a modified IgG4 CH1 region having a substitution of a lysine residue at position 196; Or (b) a modified IgG4 hinge region having a substitution of a serine residue at position 217, a glycine residue at position 220, a proline residue at position 224 or a proline residue at position 225. Preferably such IgG4 antibody further comprises a substitution of a serine residue at position 228 of the heavy chain hinge region.