REAL-TIME MONITORING DEVICE FOR QUANTITATIVE NUCLEIC ACID MEASUREMENT USING ISOTHERMAL AMPLIFICATION

摘要

Provided is a nucleic acid concentration real-time monitoring device of an isothermal amplification. According to one embodiment of the present invention, the nucleic acid concentration real-time monitoring device of the isothermal amplification comprises: a light source part for irradiating light; a loading part into which a reference tube containing only a reagent, first and second standard tubes each containing a standard sample of different nucleic acid concentration, and a plurality of object tubes each containing a plurality of object samples are loaded; a detection part for detecting an absorbance according to a time of light passing through each of the tubes; a path setting part including a first conversion member for converting a path such that the light irradiated from the light source part passes through each tube of the loading part, and a second conversion member for converting the path such that the light passing through each tube of the loading part is incident on the detection part; a driving part to move the first and second conversion members; and a control part wherein the driving part is controlled to move the first and second conversion members at the same time such that the moving distance of the light passing through the respective tubes is the same, and calculating the nucleic acid concentration of each target sample accommodated in the plurality of target tubes on the basis of the absorbance detected by the detection part. Therefore, the concentration of the nucleic acid can be immediately confirmed by measuring the absorbance.