Isothermal base dual-functional oligonucleotide containing a reporter dye and quencher for isothermal nucleic acid amplification, and nucleic acid amplification and measurement method using the same

摘要

The present invention relates to a reporter dye for isothermal nucleic acid amplification, an isothermal base dual-functional oligonucleotide containing a quencher, and nucleic acid amplification and measurement methods using the same. The present invention does not require oligonucleotides designed in addition to 4 to 6 kinds of oligonucleotides for the LAMP nucleic acid amplification reaction, and nucleic acid amplification of target gene-specific sequences for DNA and RNA. Is a method that can detect the amount of fluorescence even in real time, and can detect the amount of fluorescence even in real time, with different reporter dyes depending on the target gene or after completion of the reaction in one tube There is an effect that simultaneous multiple inspection is possible by measuring the fluorescence amount in real time.