REPORTER DYE FOR ISOTHERMAL NUCLEIC ACID AMPLIFICATION, ISOTHERMAL-BASED DUAL FUNCTIONAL OLIGONUCLEOTIDE CONTAINING QUENCHER, AND METHOD FOR NUCLEIC ACID AMPLIFICATION AND MEASUREMENT USING SAME

摘要

The invention relates to an isothermal-based dual functional oligonucleotide containing quencher and reporter dye for an isothermal nucleic acid amplification and a method for nucleic acid amplification and measurement using the same. The present invention is directed to a method capable of obviating the need for an additional oligonucleotide, in addition to four to six types of oligonucleotides for a nucleic acid amplification reaction of LAMP, detecting the amount of fluorescence according to the amplification of the nucleic acid of target gene - specific sequence for DNA and RNA, enabling the detection also after the completion of the reaction, and detecting the amount of fluorescence in real-time. Therefore, the present invention allows simultaneous multiple tests by measuring the amount of fluorescence in one tube after the completion of the reaction or in real time while varying the reporter dye according to the target gene.