METHODS FOR DETECTING NUCLEIC ACID SEQUENCE VARIATIONS AND A DEVICE FOR DETECTING NUCLEIC ACID SEQUENCE VARIATIONS USING THE SAME

摘要

The present invention relates to a next-generation sequencing method, comprising the steps of: subjecting a target sample to a plurality of times of base sequencing; collecting a plurality of base sequences including the same base sequence or a non-identical base sequence for one target sample; Comparing the base sequence of the reference sample with a plurality of base sequences, selecting a locus of the base sequence of the plurality of base sequences that is inconsistent with the base sequence of the reference sample for each of the plurality of base sequences, Determining an unmatched locus in a plurality of nucleotide sequences as candidates for nucleotide sequence variation of a target sample based on a mutation probability value for an inconsistent locus calculated by a calibrated calculation method according to an analysis, A method for detecting a mutation of a nucleotide sequence is provided.