METHOD FOR CONSTRUCTING ENGINEERED CORYNEBACTERIUM GLUTAMICUM BACTERIA HAVING HIGH PRODUCTION OF L-LYSINE

摘要

The present invention relates to a method for constructing engineered Corynebacterium glutamicum bacteria with a high production of L-lysine. Tn engineered Corynebacterium glutamicum bacterium with a high production of L-lysine is constructed by inactivating the gene NCgl1221 of Corynebacterium glutamicum, and the nucleotide sequence of the NCgl1221 gene encoding frame is shown as set forth in SEQ ID NO.1. The present invention provides a method for constructing Corynebacterium glutamicum bacterium strains having a high production of L-lysine. The extracellular secretion amount of glutamic acid is obviously reduced for the inactivation of the endogenous gene NCgl122 of the bacterium strain constructed. Compared with wild bacterial strains, the strain can produce a higher concentration of L-lysine, effectively reduce the contents of the by-product glutamic acid in the fermentation broth, and further reduce the production and purification costs as the production strain of L-lysine.