Chimeric antigen receptor (CAR) targeting T cell antigens and use in cell therapy

摘要

The present disclosure relates to genetically engineered cells, such as T cells containing the target chimeric antigen receptor. In certain embodiments, when endogenous expression of a T cell antigen is knocked down or suppressed in the T cell, the T cell targeted chimeric antigen receptor (CAR) is expressed at even higher levels. In certain embodiments, the genetically engineered cell is an immunomodulatory cell that has been genetically modified to interfere with or suppress T cell antigen expression, or if it suppresses expression of the T cell antigen, the T cell. Under conditions that increase the expression of chimeric antigen receptors compared to immunomodulatory cells in a similar state that do not modify or suppress antigen expression, the immunomodulatory cells are nucleic acids that suppress or knock down T cell mRNA expression. including. In certain embodiments, T cell antigens include, but are not limited to, CD5, CD7, and CD3.