Method for preparing a growth factor-containing platelet release

摘要

The present invention provides a platelet concentrate that is subjected to a pathogen reduction step to destroy an envelopeless virus, the platelet concentrate is subjected to an activation step to release platelet growth factors, fibrinogen-depleted humoral platelet release, and fibrinogen is recovered. A flowable mammalian platelet concentrate comprising a continuous step of subjecting the depleted flowable platelets to a second pathogen concentration reduction step to destroy the enveloped virus, sterile-filtering the platelet effluent and collecting the growth factor-containing filtrate And a method for preparing a growth factor-containing platelet release from the same. The platelet release obtained by the method of the present invention can be used as a therapeutic in regenerative medicine and for enhancing the proliferation of multilineage cells in the management of non-healing wounds and resistant ulcers. A second application is as a replacement for fetal calf serum in cell culture medium. [Selection diagram] None