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Method For Nucleic Acid Detection, Primer For Nucleic Acid Detection, And Kit For Nucleic Acid Detection

机译：核酸检测方法，核酸检测引物和核酸检测试剂盒

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摘要

[Problem] An objective of the present invention is to develop a new type of method for nucleic acid detection that can be suitably used for detection of short nucleic acids.;[Solution] The present invention provides a method for nucleic acid detection includingi) hybridizing a detection target nucleic acid 1 and a primer for nucleic acid detection 3 to form a DNA-RNA complex,ii) removing a first polynucleotide segment 301 from the primer for nucleic acid detection 3 by degrading at least a portion of the DNA-RNA complex with a nuclease 4, andiii) hybridizing the primer for nucleic acid detection 3 to a template nucleic acid 2 to perform a reaction for amplifying the template nucleic acid 2 by a polymerase 5.

机译：[问题]本发明的目的是开发一种适用于短核酸检测的新型核酸检测方法。[解决方案]本发明提供了一种核酸检测方法，包括 i）将检测目标核酸 1 和核酸检测引物 3 杂交形成DNA-RNA复合物， < ListItem id =“ ul0002-0002” number =“ 0000”> ii）通过降解至少一部分从核酸检测引物 3 的引物中除去第一个多核苷酸片段 301 。核酸酶 4 和的DNA-RNA复合物的合成）iii）杂交用于核酸检测的引物 3 与模板核酸 2 进行反应，以通过聚合酶 5扩增模板核酸 2 。 展开▼

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公开/公告号US2020263233A1

专利类型

公开/公告日2020-08-20

原文格式PDF

申请/专利权人 EIKEN KAGAKU KABUSHIKI KAISHA;
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申请/专利号US201816623544

发明设计人 SATORU MICHIYUKI;
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申请日2018-06-20

分类号C12Q1/686;C12Q1/6876;

国家 US

入库时间 2022-08-21 11:25:06

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