Glycosyltransferase engineered, polynucleotide engineered, vector, host cell, methods of producing at least one glycosyltransferase engineered to produce AT LEAST ONE EMBODIMENT OF SUCROSE SYNTHASE engineered to produce rebaudioside F, rebaudioside to and / or rebaudioside IE rebaudioside A glycosylation of a substrate, COMPOSITION, SUCHAROSIS SYNTHASE ENGINEERED, REBAUDIOSIDE M, REBAUDIOSIDE A, REBAUDIOSIDE I, E, REBAUDIOSIDE D.

摘要

the present invention provides engineered glycosyltransferase (gt) enzymes, polypeptides having gt and polynucleotide activity encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. the present invention provides engineered sucrose synthase (sus) enzymes, polypeptides having activity of sus and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. the present invention also provides compositions comprising gt enzymes and methods of using engineered gt enzymes to manufacture products with ß-glucose bonds. the present invention further provides compositions and methods for the production of rebaudiosides (for example, rebaudioside m, rebaudioside a, rebaudioside i and rebaudioside d). the present invention also provides compositions comprising sus enzymes and methods of using them. methods for producing the gt and sus enzymes are also provided.